Renal/Urology · PANCE / PANRE

Membranous Nephropathy

Adult nephrotic syndrome from subepithelial immune deposits; primary form mediated by anti-PLA2R antibodies.

Also known as: membranous nephropathy, MN, membranous glomerulonephritis, MGN, PLA2R nephropathy

Overview

An immune-mediated glomerular disease characterized by subepithelial immune complex deposition along the glomerular basement membrane, producing diffuse GBM thickening. Clinically presents as adult nephrotic syndrome.

Epidemiology

The most common primary cause of nephrotic syndrome in non-diabetic White adults. Peak incidence between ages 40-60; male predominance (~2:1). Approximately 70-80% of cases are primary (autoimmune) and the remainder are secondary to systemic disease, infection, drugs, or malignancy.

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Risk factors

  • Primary (autoimmune): anti-PLA2R or anti-THSD7A autoantibodies
  • Hepatitis B (especially in children and endemic regions), hepatitis C, syphilis, malaria
  • Solid tumors in adults >65 (lung, colon, breast, prostate, gastric) — present in ~10-25% of older patients
  • Autoimmune disease: SLE (class V), Sjögren syndrome, rheumatoid arthritis, thyroiditis
  • Drugs: NSAIDs, gold, penicillamine, captopril, anti-TNF agents
  • Stem cell or solid organ transplantation (graft-versus-host or de novo)

Pathophysiology

In primary membranous nephropathy, circulating IgG4 autoantibodies (most commonly against the M-type phospholipase A2 receptor, PLA2R; less commonly against thrombospondin type-1 domain-containing 7A, THSD7A) bind antigens expressed on podocyte surfaces. The in situ immune complexes activate complement (C5b-9 membrane attack complex), causing podocyte injury, foot-process effacement, and proteinuria. The GBM thickens around the deposits, creating the classic 'spike-and-dome' appearance on silver stain.

Clinical presentation

Symptoms

  • Insidious onset of foamy urine, dependent edema, weight gain over weeks to months
  • Fatigue, occasional gross hematuria
  • Often discovered on routine labs or with thromboembolic complication

Signs / physical exam

  • Periorbital and lower-extremity edema
  • Hypertension in 30-50% at presentation
  • Hyperlipidemia stigmata (xanthelasma) in chronic disease

Classic findings

Adult-onset nephrotic syndrome with normal complements and the highest rate of thromboembolic complications among glomerular diseases — particularly renal vein thrombosis.

Differential diagnosis

  • Minimal change disease — Abrupt onset, normal light microscopy, absent deposits, steroid-responsive
  • FSGS — Focal segmental sclerosis on biopsy; APOL1 risk in Black patients
  • Diabetic nephropathy — Long-standing diabetes, retinopathy, mesangial expansion
  • Lupus nephritis (class V) — Positive ANA/anti-dsDNA, low C3/C4, extrarenal lupus; full-house IF on biopsy
  • Amyloidosis — Monoclonal gammopathy or chronic inflammation; Congo red apple-green birefringence
  • Hepatitis-associated MN/MPGN — Positive HBsAg or anti-HCV; treat the virus

Diagnostic workup

Diagnostic criteria

Kidney biopsy shows diffuse GBM thickening on LM, granular IgG and C3 along capillary loops on IF, and subepithelial electron-dense deposits with GBM spikes on EM (Ehrenreich-Churg stages I-IV). A positive serum anti-PLA2R antibody in a patient with nephrotic syndrome and normal complements/no systemic disease may obviate biopsy in selected cases per KDIGO 2021.

Labs

  • Spot UPCR or 24-hour urine — nephrotic-range proteinuria (>3.5 g/day)
  • Serum albumin (low), lipid panel (elevated LDL and triglycerides), BMP/Cr/eGFR
  • Serum anti-PLA2R antibody (ELISA or IFA) — positive in ~70% of primary MN; quantitative titers track disease activity
  • Anti-THSD7A antibody (less commonly available; positive in ~3-5% of remaining primary cases)
  • Hepatitis B and C serologies, HIV, RPR (syphilis)
  • ANA, complements C3/C4 (typically normal in primary MN), SPEP/UPEP/free light chains
  • Age-appropriate malignancy screening: colonoscopy, mammography, low-dose chest CT, PSA

Imaging

  • Renal ultrasound — normal or enlarged kidneys; Doppler if renal vein thrombosis suspected
  • Consider CT abdomen/pelvis or PET to exclude occult malignancy in patients >65 or anti-PLA2R negative

Diagnostic algorithm

Risk category (KDIGO)DefinitionManagement
LowNormal eGFR, UPCR <3.5 g/g, albumin >3.0Supportive care, RAAS blockade, observe 6 months
ModerateNormal eGFR, UPCR 3.5-8 g/g, albumin <3.0Supportive 6 months, then consider immunosuppression if no remission
HigheGFR <60 or UPCR >8 g/g or anti-PLA2R >150 RU/mLInitiate immunosuppression (rituximab or modified Ponticelli)
Very highLife-threatening proteinuria, rapid GFR declineUrgent immunosuppression; consider cyclophosphamide-based regimen
KDIGO 2021 risk stratification for primary membranous nephropathy — guides timing and intensity of immunosuppression.

Treatment

First-line

  • Supportive care for all patients — RAAS blockade with ACE inhibitor (lisinopril, enalapril) or ARB (losartan, valsartan, irbesartan) titrated to maximum tolerated dose
  • Strict BP control (<125/75 if proteinuric)
  • Dietary sodium restriction (<2 g/day), moderate protein intake
  • Statin therapy (atorvastatin, rosuvastatin)
  • Loop diuretic (furosemide, torsemide, bumetanide) for edema
  • Risk-stratify (low/medium/high/very high) per KDIGO using proteinuria, eGFR, albumin, and anti-PLA2R titer; observe low-risk patients for 6 months before initiating immunosuppression

Primary MN, moderate-to-high risk (persistent nephrotic-range proteinuria after 6 months, declining GFR, or very high anti-PLA2R titer)

  • Rituximab — increasingly preferred first-line per KDIGO 2021 (MENTOR trial)
  • Modified Ponticelli regimen — alternating monthly cyclophosphamide and corticosteroids for 6 months
  • Calcineurin inhibitor (cyclosporine, tacrolimus) — alternative; high relapse rate after withdrawal

Secondary MN

  • Treat the underlying cause: antiviral therapy for HBV/HCV, oncologic treatment for malignancy, withdraw causative drug, immunosuppression for SLE
  • Avoid empiric immunosuppression without identifying the cause

Second-line / adjunct

  • Anticoagulation — prophylactic if serum albumin <2.5-2.8 g/dL with additional risk factors; therapeutic for confirmed thromboembolism (warfarin or DOAC depending on severity)
  • Obinutuzumab or ofatumumab in rituximab-refractory disease
  • Stem-cell-based therapies and CAR-T in investigational protocols

Complications

  • Renal vein thrombosis (highest incidence among glomerular diseases — up to 30%); also DVT and PE
  • Progression to CKD/ESKD in untreated disease (rule of thirds: 1/3 spontaneous remission, 1/3 persistent proteinuria, 1/3 progressive)
  • Infection from urinary loss of immunoglobulins; pneumococcal and influenza vaccination
  • Cardiovascular disease from chronic dyslipidemia and hypertension
  • Steroid and cyclophosphamide toxicity in patients on Ponticelli regimen

PANCE pearls

  • Membranous nephropathy carries the highest rate of renal vein thrombosis among glomerular diseases — sudden flank pain, gross hematuria, or worsening proteinuria should prompt evaluation with Doppler or CT venography.
  • Anti-PLA2R titers correlate with disease activity and predict response to therapy; a falling titer often precedes clinical remission.
  • Malignancy-associated MN typically presents in patients >65; staining of tissue for tumor-associated antigens (NELL-1, exostosin 1/2) can help.
  • Rule of thirds for spontaneous course remains a useful counseling framework.
  • Hepatitis B is the leading global cause of secondary MN, especially in pediatric populations.

References

  • KDIGO 2021 — KDIGO 2021 Clinical Practice Guideline for the Management of Glomerular Diseases (Kidney Int 2021;100:S1-S276)
  • MENTOR Trial — Fervenza FC et al. Rituximab or cyclosporine in the treatment of membranous nephropathy (NEJM 2019;381:36-46)
  • PLA2R — Beck LH et al. M-type phospholipase A2 receptor as target antigen in idiopathic membranous nephropathy (NEJM 2009;361:11-21)
  • STARMEN — Fernandez-Juarez G et al. Tacrolimus-rituximab vs cyclophosphamide-steroids in membranous nephropathy (Kidney Int 2021;99:986-998)

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