Reproductive · PANCE / PANRE

Trichomoniasis

Sexually transmitted protozoal infection — frothy yellow-green discharge, strawberry cervix; treat patient and partners.

Also known as: trichomoniasis, trich, Trichomonas vaginalis, TV

Overview

Sexually transmitted infection caused by the flagellated protozoan Trichomonas vaginalis, producing vulvovaginitis in women and urethritis (often asymptomatic) in men.

Epidemiology

Most common nonviral STI globally; ~2 million US cases/year. Disproportionate prevalence in Black women (~10%) and incarcerated populations. Frequently coexists with other STIs.

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Question 1ReproductiveMedium
A 26-year-old woman presents with 5 days of frothy, malodorous yellow-green vaginal discharge, vulvar itching, and burning with urination. She is not pregnant and has had two new partners in the past 2 months. On speculum exam there is vaginal erythema and punctate cervical hemorrhages. Vaginal pH is 5.5, and saline wet mount shows motile, pear-shaped flagellated organisms. Which of the following is the most appropriate initial management?
  • AMetronidazole 250 mg orally three times daily for 7 days
  • BMetronidazole 500 mg orally twice daily for 7 days
  • CMetronidazole 2 g orally as a single one-time dose
  • DMetronidazole 0.75% vaginal gel nightly for 5 days
Reveal answer & full explanation
Correct answer: B — Metronidazole 500 mg orally twice daily for 7 days
  • AMetronidazole 250 mg orally three times daily for 7 days
  • BMetronidazole 500 mg orally twice daily for 7 days
  • CMetronidazole 2 g orally as a single one-time dose
  • DMetronidazole 0.75% vaginal gel nightly for 5 days

Why Metronidazole 500 mg orally twice daily for 7 days is correct

  • The frothy yellow-green discharge, strawberry cervix (punctate hemorrhages), pH >4.5, and motile flagellated trichomonads on wet mount confirm Trichomonas vaginalis vaginitis.
  • Per CDC 2021 STI treatment guidelines, first-line therapy for women is metronidazole 500 mg PO BID for 7 days; this multidose regimen replaced the single 2 g dose in women because the 7-day course produces lower recurrence.
  • Sexual partners should also be treated, and the patient should be re-tested within 3 months given high reinfection rates.

Why the others are wrong

  • Metronidazole 2 g orally as a single one-time dose — correct drug class, but the single dose is now first-line only for men; in women it has higher recurrence and is no longer preferred.
  • Metronidazole 250 mg orally three times daily for 7 days — a superseded lower-dose oral regimen that delivers less total drug than the currently recommended course; CDC specifies 500 mg twice daily for 7 days in women.
  • Metronidazole 0.75% vaginal gel nightly for 5 days — topical metronidazole treats bacterial vaginosis but does not reach therapeutic levels in the urethra and periurethral glands where trichomonads persist, so cure rates are poor and it is explicitly not recommended for trichomoniasis.
Question 2ReproductiveMedium
A 25-year-old woman at 26 weeks' gestation reports a 1-week history of frothy, malodorous yellow-green vaginal discharge with vulvar burning. On speculum exam, the cervix shows punctate hemorrhages and vaginal pH is 5.5. A nucleic acid amplification test confirms Trichomonas vaginalis, and she begins metronidazole 500 mg PO twice daily for 7 days. Which of the following complications is this infection most likely to contribute to in this patient?
  • ANeonatal sepsis
  • BPreterm delivery
  • CPostpartum hemorrhage
  • DPlacental abruption
Reveal answer & full explanation
Correct answer: B — Preterm delivery
  • ANeonatal sepsis
  • BPreterm delivery
  • CPostpartum hemorrhage
  • DPlacental abruption

Why Preterm delivery is correct

  • Trichomoniasis in pregnancy is associated with adverse obstetric outcomes — preterm birth, premature rupture of membranes, and low birth weight — driven by ascending genital inflammation.
  • In a confirmed, symptomatic pregnant patient, preterm delivery is the highest-yield trichomoniasis-related complication to recognize; the CDC recommends treating symptomatic infection in pregnancy.
  • Note that while symptomatic infection is treated, antibiotic therapy does not consistently reduce the preterm birth risk, so it remains the key associated outcome.

Why the others are wrong

  • Placental abruption — abruption is driven by chronic or gestational hypertension, trauma, cocaine use, and smoking; T. vaginalis is not a recognized cause, and she has no bleeding, uterine tenderness, or elevated blood pressure.
  • Neonatal sepsis — early-onset neonatal sepsis follows group B streptococcal or coliform colonization with chorioamnionitis; Trichomonas is a lower-tract protozoal infection and is not an established cause of invasive neonatal infection.
  • Postpartum hemorrhage — bleeding after delivery reflects uterine atony, retained placental tissue, genital tract laceration, or coagulopathy, none of which trichomoniasis produces.
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Risk factors

  • Multiple or new sex partners
  • Inconsistent condom use
  • Coexisting STIs, especially BV and HIV
  • Incarceration
  • IV drug use
  • Lower socioeconomic status / limited healthcare access

Pathophysiology

T. vaginalis adheres to vaginal epithelium via surface adhesins and produces cytotoxins, causing epithelial inflammation, micro-ulceration, and increased risk of HIV transmission/acquisition. The organism cannot survive outside the urogenital tract.

Clinical presentation

Symptoms

  • Frothy, malodorous yellow-green discharge
  • Vulvar and vaginal pruritus, burning
  • Dyspareunia, dysuria
  • Postcoital bleeding
  • ~50% of women and most men are asymptomatic

Signs / physical exam

  • Frothy yellow-green discharge
  • Vulvovaginal erythema
  • 'Strawberry cervix' (punctate hemorrhages) — pathognomonic but seen in only ~2-5%
  • Vaginal pH >4.5

Classic findings

Sexually active woman with frothy malodorous discharge, vulvar pruritus, and strawberry cervix on colposcopy or speculum exam.

Differential diagnosis

  • Bacterial vaginosis — Thin gray discharge, fishy odor, clue cells; no motile organisms
  • Candidal vulvovaginitis — Thick white discharge, intense pruritus, normal pH; KOH shows yeast
  • Gonorrhea / chlamydia cervicitis — Mucopurulent cervical discharge; NAAT positive
  • Atrophic vaginitis — Postmenopausal, dryness; thin pale mucosa
  • Foreign body / retained tampon — Malodorous discharge resolving with removal

Diagnostic workup

Labs

  • NAAT (nucleic acid amplification test) — preferred; high sensitivity/specificity
  • Wet mount microscopy — motile, pear-shaped flagellated trichomonads (~50-70% sensitivity)
  • Point-of-care antigen tests (OSOM Trichomonas, Affirm VPIII) — better than wet mount, suitable for clinical setting
  • Culture (Diamond medium) — high specificity but slow
  • Vaginal pH >4.5, often positive whiff test
  • Test for other STIs: GC/CT, HIV, syphilis, HBV

Diagnostic algorithm

FeatureTrichomoniasisNotes
Causative organismTrichomonas vaginalis (flagellated protozoan)Only STI listed here that is a parasite
Classic dischargeFrothy yellow-green, malodorousMay also be thin and yellow
Exam clueStrawberry cervix (punctate hemorrhages)Present in only 2-5% but pathognomonic
Vaginal pH>4.5Same as BV; differentiates from candidiasis
DiagnosisNAAT preferred; wet mount fast but ~50-70% sensitiveMotile trichomonads on wet mount
Treatment (women)Metronidazole 500 mg PO BID × 7 daysReplaced single 2 g dose (2021 CDC update)
Treatment (men)Metronidazole 2 g PO × 1Tinidazole 2 g × 1 acceptable
Partner treatmentYes — all partnersExpedited partner therapy where legal
PregnancyTreat with metronidazoleAvoid tinidazole
Trichomoniasis at-a-glance: presentation, diagnosis, treatment.

Treatment

First-line

  • Women: metronidazole 500 mg PO BID × 7 days (CDC 2021 — replaced single 2 g dose for women based on trial data showing lower recurrence with 7-day regimen)
  • Men: metronidazole 2 g PO × 1 dose
  • Alternative: tinidazole 2 g PO × 1 dose

Pregnancy

  • Treat symptomatic infection — metronidazole 500 mg PO BID × 7 days (or 2 g × 1 dose)
  • Metronidazole crosses placenta but evidence does not support teratogenicity; benefits outweigh risks
  • Avoid tinidazole in pregnancy
  • Counsel about preterm birth risk; treatment does not consistently reduce this risk

HIV-positive women

  • Metronidazole 500 mg PO BID × 7 days (single-dose regimen less effective)
  • Re-screen at 3 months
  • Important to treat — increases viral shedding and transmission

Second-line / adjunct

  • Treatment failure: re-treat with metronidazole 500 mg PO BID × 7 days; if persistent, tinidazole 2 g PO daily × 7 days; if still refractory, susceptibility testing and high-dose tinidazole
  • Avoid alcohol during and for 24 hours after metronidazole (72 hours after tinidazole) — disulfiram-like reaction
  • All current sexual partners should be treated regardless of symptoms (expedited partner therapy where legal)
  • Re-test all women within 3 months due to high reinfection rate

Complications

  • Increased risk of HIV transmission and acquisition
  • Postpartum endometritis, post-hysterectomy cellulitis
  • Pregnancy: preterm birth, premature rupture of membranes, low birth weight
  • PID (less common than with GC/CT)
  • Male infertility (rare; epididymitis/prostatitis)

PANCE pearls

  • T. vaginalis is the only STI for which the CDC switched from a single-dose to multidose regimen for women based on superiority data.
  • All sexual partners should be treated — even asymptomatic ones — to prevent reinfection.
  • Wet mount is rapid but insensitive; NAAT is the diagnostic test of choice when available.
  • Re-screen women at 3 months due to high reinfection rates (~17%).
  • Strawberry cervix is highly suggestive but infrequent — its absence does not exclude trichomoniasis.

References

  • CDC STI Guidelines 2021 — CDC Sexually Transmitted Infections Treatment Guidelines, 2021 (MMWR Recomm Rep 2021)
  • Kissinger et al. 2018 — Single-dose Metronidazole vs 7-day Treatment for Trichomonas in Women (Lancet Infect Dis 2018)
  • ACOG PB 215 — ACOG Practice Bulletin No. 215: Vaginitis in Nonpregnant Patients

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